Nosema ceranae then and now: comparing DNA sequences of this parasite between 1994 and the present.
1 Warsaw University Of Life Sciences, Faculty of Veterinary Medicine, Department of Pathology and Veterinary Diagnostics, Laboratory of Bee Diseases
It is thought that Nosema ceranae is a new threat to the European honeybee and that due to the lack of host adaptation to its presence, it can cause a very high mortality, because the bee has not had time to evolve any protective barriers. In Poland, we found N. ceranae in bees collected two decades ago and stored ever since. Some researchers think that the high mortality may be a result of genetic changes that this parasite might have undergone, which might have made it more virulent. In this study we attempt to compare the genetic material of N. ceranae obtained from 6 historical bee samples (collected in 1994 from 6 Polish apiaries and frozen ever since), with 9 bee samples collected currently, also confirmed to be infected with N. ceranae (also from different Polish apiaries). The DNA of the parasite will be extracted according to OIE recommendations, and the pre-sequencing PCR will be carried out using NosaRNAPol-F2 and NosaRNAPol-R2, as recommended for sequencing by Gisder et al. (2013). The product will be enzymatically cleaned and sent to an external facility for the sequencing procedure. We want to check if there is any differences between N. ceranae from the early 1990’s and now. We hope that the next step of our research will be to investigate if the possible genetic changes in Nosema ceranae could influence its virulence.