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Bee Research Institute Dol
Dol 94
Libcice nad Vltavou
252 66
Czech Republic
+450 220 940 480 line 124
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Case report of European foulbrood in the Czech Republic: a new outbreak after 40 years.

Martin Kamler1, Jan Tyl1, Jan Hubert2, Marta Nesvorná2, Alena Vaníčková3, Jiří Merta3, Dalibor Titěra1

1Bee Research Institute Dol, Dol 94, Libcice nad Vltavou 252 66, Czech Republic

2Crop Research Institute, Drnovská 507, Prague 6 - Ruzyne, Czech Republic

3Regional Veterinary Administration, Hradec Kralove Region, Jana Cerneho 370/40, Hradec Kralove 503 41, Czech Republic

The European countries report new cases of the European foulbrood (EFB) in honeybee (Apis mellifera) colonies. The disease is caused by entomopathogenic bacteria Melissococcus plutonius. The outbreak of EFB was recently confirmed in the Czech Republic. Based on the record it is approximately after of 4 decades of the last outbreaks in this country.

Two apiaries of one beekeeper were suffered EFB during June and August 2015. In June 2015, quick weaknesses of honeybee colonies have been recorded in the first apiary by the beekeeper. Scattered brood pattern and decreased number of honeybee larvae were observed. The melted larvae and hive bees were collected from every apiary and DNA was extracted from 10 individuals. The PCR using specific primers confirmed M. plutonius in larvae, open pupae and adults of A. mellifera in all beehives in the apiary (n=7). The second apiary is around 5 km far. Clinical symptoms were observed in 5 of 7 inspected colonies in the apiary (n=11). PCR examination confirmed M. plutonius in honeybee larvae and adults in colonies with clinical symptoms.

The EFB is notifiable disease in the Czech Republic. 5 km protection zone is established around apiary site with confirmed EFB. Approximately 600 – 700 colonies are present in actual protection zone. Colonies will be inspected during survey for clinical symptoms and sampled for wax debris to analyze M. plutonius. Results, possible source of infection and epidemiology of this outbreak will be discussed in poster at COLOSS conference in October 2015. The study is supported by the project QJ1310085 of the Czech Ministry of Agriculture.


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