PEREZ DE LA ROSA
MOLECULAR DIAGNOSIS OF MAIN VIRAL DISEASES IN REGIONS OF THE CENTER AND SOUTHEAST OF MEXICO.
aCentro Nacional de Servicios de Constatación en Salud Animal (CENAPA), SENASICA, Carretera Federal México-Cuautla No.8534. Col. Progreso, Jiutepec, Morelos, México. C.P.62550.
Diseases transmitted by viral pathogens that infect the honey bees have been reported in many countries, however, in Mexico there is a limited number of reports about main viral pathogens that affect the honey bees. This study was conducted for the diagnosis of main honey bee viruses: Sacbrood virus (SVB), Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Israeli acute paralysis virus (IAPV) and Kashmir bee virus (KBV), in samples of honey bees larvae of different apiaries from regions of the Center and Southeast of Mexico. Results obtained of the RT-PCR technique, demonstrated the presences of the viruses DWV, SBV, BQCV, and ABPV in honey bees larvae, from regions of the Central and Southeast of Mexico, however, no samples was tested positive for the viruses IAPV and KBV.Sequences of the viruses SBV, DWV, ABPV and BQCV showed more than 99% homology with sequences reported in GenBank, of the National Center for Biotechnology Information, NIH. The present study proves evidence that main honey bee viruses (DWV, SBV, BQCV and ABPV), are circulating in honey bee larvae of apiaries from regions of the Central and Southeast of Mexico. In Mexico the real impact of viral pathogens infection in honey bees is uncertain, is important monitoring the presence and spread of these viruses to know the impact of them in the health of honey bees colonies in Mexico. In conclusion, these results demonstrate the importance of developing and maintaining a program of surveillance and control of main viral pathogens in honey bees of Mexico.