Concentrations of total proteins and glucose in brood of honeybee colonies fed with food additives
Department for Biology and Pathology of Fish and Bees, University of Zagreb Faculty of Veterinary Medicine, Heinzelova 55, 10 000 Zagreb, Croatia
Ministry of Agriculture, Veterinary and Food Safety Directorate, Planinska 2, Zagreb, Croatia
Laboratory for Molecular Neuropharmacology, Institute Ruđer Bošković, Bijenička cesta 54, 10 000 Zagreb, Croatia
Agriculture advisor servis, Stilinovićeva 17, 10431 Sv. Nedjelja, Croatia
Honeybee colonies are exposed to numerous unfavourable threats from various diseases and environmental factors. Nosemosis type C is primarily a disease of the digestive tract in adult honey bees caused by the microsporidian Nosema ceranae. Since there is no authorized veterinary medicinal product for the treatment of the same and antibiotics are currently forbidden throughout the European Union due to possible determination residue levels in honey, it is important to find alternative treatments to control Nosemosis type C. The food supplements are fed to honey bees to supply the nutritive requirements of colonies in areas and during periods when natural food sources (pollen, nectar or honey) are inadequate or not available and they can improve the body functions, digestion, and help better utilization of food and development of their immune system.
The aim of this study is to examine the effect of repeated applications of various food supplements (including herbs, minerals and protein ingredients) on the biochemical and histochemical indicators, including concentrations of total proteins and glucose. Mentioned parameters were determinated in five day old larvae samples originated from colonies repeatedly fed with different food additives (Nozevit, NozevitPlus, Eko ZeoPet, BeeWell AminoPlus, BPC-157). Additional feeding was performed according to manufacturerʼs instructions for use, respectively, and for Eko ZeoPet and BPC-157 was recalculate on the basis of described use in human and veterinary medicine. Sampling were performed 10th, 20th and 30th day from initial feeding. Total protein concentrations were measured using Bradford protein assay spectroscopic analytical procedure in suspension of larvae tissue. Glucose was measured using commercial Glucose (GO) Assay Kit. For the purpose of describing glycogen and/or oxidable dioles content we used the Periodic Acid-Schiff Reaction (PAS). Control group of honeybee colonies were fed with sugar syrup without any food supplement.
Results for total proteins mean values concentrations ranges as follows: Nozevit 0.087-0.120; NozevitPlus 0.094-0.137; Eko ZeoPet 0.088-0.120; BeeWell Aminoplus 0.087-0.114; and BPC-157 0.091-0.114 (mg/mg larvae tissue), respectively. Among these results and also glucose concentrations isn’t found statistically significant difference, as well as between experimental and control group of honeybee colonies.