Announce: COLOSS Workshop, Nov 2010

Topic: Standardized methods for honey bee rearing in laboratory cages

Dear Coloss colleagues,

we are planning to hold the Workshop on "Standardized methods for honey bee rearing in laboratory cages" at the end of November 2010.

However, considering that this workshop was planned for last spring and then cancelled due to low number of participants, we are now collecting expressions of interest before definitive organisation. If interest in the topic is low then the budget may be altered in favour of other activities.

Below you may read the aims and program of the Workshop.

I kindly ask colleagues who think they will take part in the workshop to inform me by email at this address by Septmber the 17th. I remind you that, as in the other workshops, reimbursement will cover travel expenses only.

Best regards,
Cecilia Costa

Motivation for workshop:
Hoarding cages are used for many different kinds of tests, such as evaluation of, toxicity, bee vitality, longevity, development of disease, efficacy of medicaments, synergic effects of several factors, but scientists conducting independent experiments on these subjects often make different choices concerning use of this tool (hoarding cage type, temperature, quality and amount of food provided etc), infection/infestation procedure (storage of the pathologic material, method of inoculation, honey bee age etc.) and data recording (post-infection/post-infestation age, examined organs, behavioural observations, longevity assessment etc.).
 
Even though the above differences are not sufficient to invalidate some of the trials, obvious problems show up when attempts to compare the inter-experiment results are made. This points out that efforts to standardise the working procedures are very much needed because rearing and observing honey bees in the laboratory is an important common tool used in many research areas.
 
This workshop is in line with the present need to extend standardization efforts to the different  research fields. The final aim is to improve the comparability of experimental results and to produce standard protocols specific for different tests that could be included in the BEE BOOK.

Scientific programme:
 
The following issues will be discussed: 
 
1 – Observations on ADULT BEES 
1a – Stress/wellbeing assessment
1b – Longevity tests
1c – Toxicity of medicaments for the bees
1d – Toxicity of environmental contaminants (pesticides etc.)
 
2 – Observations on PATHOGENS 
2a – Development of infections
2b – Efficacy of medicaments for the bees
 
3 – Comparisons: REARING CONDITIONS AND MATERIAL 
3a - Kind of cage (material, size, hygiene)
3b – Incubation conditions (T, RH, light, precision required for each)
3c - Origin and age of bees 
3d - Practical procedures of bee handling (bee collection and anaesthetization)
3e - Kind and mode of feeding 
3f – Administration modes of the tested factors (individual / collective inoculation / intoxication)
3g - Duration of test
 
4 – STATISTICS 
4a - Number of bees per cage
4b - Number of repetitions and replicates
4c – Experimental setup (problem of autocorrelation in longevity tests)
4d – Observation classes (definition of “dead bee”)
 
5 - RELATIONSHIP BETWEEN LAB AND FIELD RESULTS




Dr Cecilia COSTA 
Consiglio per la Ricerca e la sperimentazione in Agricoltura
Unità di Ricerca di Apicoltura e Bachicoltura 
CRA-API 
Via Fratelli Rosselli, 80
42100 Reggio Emilia (RE);
Via di Saliceto, 80
40128 Bologna (BO)
Italia
cecilia.costa@entecra.it
http://www.entecra.it
Tel. +39-0522-285532; +39-051-353103
Fax. +39-0522-285532; +39-051-356361