Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C?



The study was conducted to investigate the effect of
Lactobacillus rhamnosus (a commercial probiotic) and inulin
(a prebiotic) on the survival rates of honeybees infected and
uninfected with Nosema ceranae, the level of phenoloxidase
(PO) activity, the course of nosemosis, and the effect on the
prevention of nosemosis development in bees. The cells of
L. rhamnosus exhibited a high rate of survival in 56.56 %
sugar syrup, which was used to feed the honeybees. Surprisingly,
honeybees fed with sugar syrup supplemented with a
commercial probiotic and a probiotic+prebiotic were more
susceptible to N. ceranae infection, and their lifespan was
much shorter. The number of microsporidian spores in the
honeybees fed for 9 days prior to N. ceranae infection with
a sugar syrup supplemented with a commercial probiotic was
25 times higher (970 million spores per one honeybee) than in
a control group fed with pure sucrose syrup (38 million spores
per one honeybee). PO activity reached its highest level in the
hemolymph of this honeybee control group uninfected with
N. ceranae. The addition of probiotics or both probiotics and
prebiotics to the food of uninfected bees led to the ~2-fold
decrease in the PO activity. The infection of honeybees with
N. ceranae accompanied an almost 20-fold decrease in the PO
level. The inulin supplemented solely at a concentration of
2 μg/mL was the only administrated factor which did not
significantly affect honeybees’ survival, the PO activity, or
the nosemosis infection level. In conclusion, the supplementation
of honeybees’ diet with improperly selected probiotics
or both probiotics and prebiotics does not prevent nosemosis
development, can de-regulate insect immune systems, and
may significantly increase bee mortality.

 

Ptaszyńska A.A., Borsuk G., Zdybicka-Barabas A., Cytryńska M., Małek W. 2015. Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C? Parasitology Research, DOI: 10.1007/s00436-015-4761-z

2015 Parasit Res Are.pdf
http://link.springer.com/article/10.1007%2Fs00436-015-4761-z#