3.3.4.2. Isolation of the RCC and CA

1.   Sever the section of AO-ES just anterior to where the complex is attached.

2.   Remove the whole mass from the head.

3.   Grasp the AO-ES rather than the RCC to avoid damaging the CA and CC.

4.   Use forceps to gently push the complex off of the AO-ES, leaving an isolated    RCC.

5.   Transfer the AO-ES/RCC to an incubation medium if dissecting to measure the rate of juvenile hormone biosynthesis by the paired corpora allata (see section 3.5. of the BEEBOOK paper on physiology and biochemistry (Hartfelder et al., 2013)) or another appropriate physiological liquid (e.g. Ringer solution, see Table 1 of the BEEBOOK chapter on cell cultures (Genersch et al., 2013)) to prevent desiccation.

For some assays, it is desirable to leave attached tracheal elements intact to enhance the buoyancy of the complex (Fig. 45.4.). Avoid grasping the glands with forceps as the endocrine tissue is readily damaged, and often the glands will adhere to the forceps and become difficult to dislodge. The CA or entire RCC can be moved between different media by suspending the glands in a droplet of liquid held by a small wire hoop.