126.96.36.199. Displaying the reproductive organs (Plate 16B)
1. Grip the ventriculus with forceps and stretch it, drawing it towards the rear end of the abdomen.
2. Cut the canal in front of the crop and through the rectum.
3. Remove the alimentary canal.
This must be done without damaging the ovaries.
4. Very carefully slip a needle under one of the ovaries and lift it, turning it slightly outwards.
The tracheae which bind and suspend the ovary will now be seen stretched.
5. Break the tracheae with a needle held in the other hand, until the ovary is freed sufficiently to lie over to the side, as shown in the plate.
6. Repeat this operation with the other ovary.
7. Lower the sides of the body wall near the tip of the abdomen.
8. Flush out the body cavity with the pipette.
9.1. the paired oviducts, which converge to the root of the sting, where they join
9.2. the median oviduct;
9.3. the spermatheca;
9.4. the spermathecal gland and investment of silvery tracheae;
9.5. the acid gland of the sting, which is much longer than the worker's;
sting apparatus is firmly anchored and cannot be lifted out as it can from a
worker dissection. The shaft of the sting is curved, not straight like the worker's.
Queens used for dissection are often discarded ones, a year or two old, sometimes older. In these old queens the fat body and Malpighian tubules are discoloured by the accumulation of waste products (this can be seen in the pericardial fat cells; it is not necessary to spoil a good dissection by removing the viscera to expose the fat cells on the floor of the abdomen).