3.6.1. Dissection of the larva (Plate 19)

1. Choose large coiled larvae.

In the fresh state they are too fragile to handle, while if they are fully hardened the fat cells form a solid mass which cannot be broken up without destroying the viscera. They must therefore be hardened:

2. Immerse in formol-alcohol for only two or three days before dissection.

In any case, the removal of the fat is a rather tedious process, requiring care and patience to take it away piecemeal.

3. Lay the coiled larva on the side since it cannot be straightened out for dissection.

4. Remove the body wall using scissors.

5. Disengage the fat in small fragments.

If this is done successfully, the structures shown in Plate 19 will be revealed. The plate shows a drone larva, more convenient for this work on account of its larger size. The ovaries are very small in worker larvae, much larger in queen larvae; they occupy the same position as the testes, which are still larger.