11.2.3. Attaching the transponder

The transponders can be attached to the bee thorax in the following way, which is a further development of the method reported by Capaldi et al. (2000):

1. Tag individual bees with a number plate (see 2.3; Fig. 1).

2. The base of the transponder is also a number tag which carries in its centre a tiny piece of a tubing (Micro-katheter PVC Chemies Schlauch, www.rct-online.de) into which the wire fits tightly (Fig. 23A).

3. Glue this tube to the number tag with acrylic superglue.

4. Fix the other side of the number tag to a fitting piece of double sided sticky tape (Pattex, Henkel).

5. Keep the protecting cover of the double sided sticky tape on the other side.

6. Remove the cover immediately before fixing the transponder to the number tag on the thorax of the bee (Fig. 24).

7. Now push the experimental bee with a stopper against the net of a queen marking device (Fig. 24B) and align it such that the number tag on the thorax appears in a window of the net.

8. Finally, push gently the fresh gluing surface of the double sticky tape against the number tag on the bee.

Fig. 24. Fixing the transponder to the thorax of a test bee. A. First the cover of the double sticky tape at the base of the transponder is removed. B. Then the bee is caught with a marking device and pushed with a stopper against a net. The transponder is caught with fine forceps at the tube and pushed onto the number tag of the bee (Photos: Christoph Grüter).

1293PN revised Fig 24

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