14.4.1. Preparing the bees

1. Mark a newly recruited forager from a sucrose feeder with a highly visible colour spot on the thorax or abdomen.

2. Train the marked bee to find a droplet of a high-concentrated sucrose solution (e.g. 50 %) on the setup (for more details see Fig. 38).

3. Use landmarks (e.g. coloured plates) to guide the marked bee to the sucrose droplet or transfer the bee on a tooth pick from the feeder to the setup.

In the following visits, the bee usually remembers the location of the high-sucrose reward.

4. After three visits to the setup, start with the conditioning procedure.

A visit is defined as one foraging flight between the hive and the setup.