6.2.4. One-way shuttle box

One strategy to eliminate some of the problems associated with a two way shuttle box is to use a "one-way" shuttle box. A one-way shuttle box gets its name because the animal is always moving in the same direction. This device eliminates the problem of shuttle box dimensions, bees potentially walking on the ceiling of the apparatus, and accommodates a variety of bee species without much effort. In one version of this device a bee is tethered to a pole in the centre of a 50.8 mm square grid using a stiff wire. One end of the wire is attached to the thorax with wax and the other end to the pole. The wire is secured to the pole by inserting it into a small hole. The location of the hole is approximately the height of a honey bee enabling the bee to easily turn the pole while it circumnavigates the space. To provide a cue for the bee that it enters a new section of the space (i.e. a new compartment), small, thin, 2mm plastic strips can be secured to the surface that the bee must step over. For example, if a 4 section shuttle box is required for a 50.8mm space, plastic hurdles are placed every 12.7 mm. Infrared photocells can be placed near the plastic hurdles to automate response detection. To provide electric shock a grid can be used or antistatic foam (Abramson et al., 2004).