3.2. Cultivation of insect cell lines

Cultivation of insect cell lines is straightforward. Normally, they are maintained at room temperature (20-27°C) without CO2 allowing cultivation of these cells “in the desk drawer”. However, suitable cooling incubators (e.g. Heraeus BK6160 through Thermo Fisher Scientific) which keep a defined temperature are recommended. To avoid evaporation of the medium during cultivation, cooling should not be accomplished through air ventilation. Each cell line, when purchased from a cell culture collection, will be accompanied by a data sheet giving all necessary information concerning the medium for cultivation, how and when to subculture, doubling time, cell harvest, and storage conditions. It is advisable to first follow these instructions before adapting these protocols to experimental needs.