6.2.1. Isolation of organs/tissues

Incomplete isolation of the gland from other organs and tissues may negatively affect the glandular performance. For example, residues of fat body or other destroyed tissues can contribute degradation factors such as proteases, RNAases, etc. These can severely reduce glandular activity and thus obscure the studied effects. For example, Dufour’s glands should be completely separated and cleared from the sting and the poison gland whilst mandibular glands need to be separated and cleared from labial, hypopharyngial glands and muscles. While dissecting the labial glands, the head glands need to be carefully separated from the hypopharyngeal gland and the thoracic glands for precise separation from the thoracic muscles.