6.2.5. Incubation conditions
Incubation conditions (temperature and humidity) should be defined based on preliminary studies reflecting normal physiological and environmental conditions. Humidity should be maintained to prevent water evaporation and media concentration during the incubation process. Temperature can be maintained by incubating the samples in temperature controlled conditions (water bath or temperature controlled dry blocks). To prevent evaporation, the incubation vials must be kept sealed with protective cover or Parafilm. The number of glands (1 or 2 glands) per incubation may vary and depend mainly on the sensitivity of the detection method and on their secretion rate.