6.2.7. Extraction of pheromone
Extraction of biosynthesized pheromonal components is the final step of an in vitro incubation. It involves extraction of the product from the incubation medium by an appropriate solvent and this depends on the chemical properties of the product. Recommended solvents for extracting pheromonal components are pentane/hexane/heptane or dichloromethane. The first group of solvents is especially suited for non-polar compounds such as hydrocarbons while dicholoromethane, a moderately polar solvent, efficiently extracts components such as aldehydes and ketones.