5.2. Computer-assisted digital image analysis

Computer-assisted digital image analysis can be used to directly measure surface area of comb occupied by bees or other colony resources such as open brood, sealed brood, or pollen. There are two kinds of data output: 1. direct surface measurements (cm2 or dm2) of target (Cornelissen et al., 2009) (5.3.) and; 2. ratio of surface target relative to total comb surface (Yoshiyama et al., 2011) (5.4.). In the case of 1., it is possible to convert surface to units of bees or cells using conversion values in Table 2.