5.5. Ratio of target surface relative to total comb surface area

This method, also applying ImageJ, yields the ratio between selected area and the total comb area. Prior to computer analysis, each digital image of a comb side is digitally edited by the investigator to delineate comb areas of target resource, e.g. capped brood area, open brood, pollen, or honey with unique identifying colours. In Fig. 12, this application is outlined.

Fig. 12. Outline of method of Yoshiyama et al. (2011) for determining ratio of target surface : total comb surface. It is used with the OECD colour codes in Table 3.Figure 12

Table 3. Colour- and number-coding of cell contents according to OECD (2007).

Brood stages / stores

Colour

Number

empty cells

Brown

0

eggs

Blue

1

young larvae (1-3 days)

Green

2

older larvae (4-6 days)

Red

3

pupae (capped brood)

Yellow

4

nectar

orange

5

pollen / bee bread

deep pink

6

dead larvae / pupae

dark salmon

7

not characterized (nc)

White

8