3.3. Fixation, paraffin embedding, sectioning and mounting

In histological analyses, paraffin sectioning is one of the most widely used techniques, and many good protocols are available (e.g. Barbosa (1974) or Presnell et al. (1997)). One can refer to these protocols to learn the principles and details of different techniques. Here, we describe the conditions useful for preparing paraffin sections of the honey bee gut.