4.4.1. Optimal growth conditions

  1. Atmosphere can be aerobic or anaerobic
  2. Temperature of 35-37°C
  3. Culture media: tryptone soy broth agar (Oxoid; Basingstoke, Hampshire, UK), tomato juice agar  (Oxoid; Basingstoke, Hampshire, UK), Rogosa agar (Merck), and MRS (de Man-Rogosa-Sharpe) agar (Oxoid; Basingstoke, Hampshire, UK), Lactobacillus Carrying Media (Efthymiou and Hansen, 1962) and MRS broth supplemented with 0.5% L-cysteine or 20% fructose (Olofsson & Vásquez, 2008; Forsgren et al. 2010).