2.2.1. Simple saline formula

The simple saline diluent formula (Table 1) can be used for same-day semen collection and insemination (Williams and Harbo, 1982). An alternative, very basic physiological saline solution is adequate (0.9% NaCl, 0.1% glucose and antibiotic).

Table 1. Simple saline formula based on Williams and Harbo, 1982. Add all of the ingredients to a volumetric flask then add distilled water to make up a final volume of 100 ml.

Ingredient

g ingredient / 100 ml final volume   

Dihyrdostreptomycin 

0.25

Glucose

0.10

L-lysine

0.01

L-arginine

0.01

L-glutamic acid

0.01

Trizma HCL

0.35

Trizma base

0.35

NaCl

1.11