5.4.2. Drone seminal vesicles

  1. Cut off the drone abdomen and immediately freeze head/thorax at -80°C for any further analyses.
  2. Pin abdomen down in petri dish and dissect out the seminal vesicle:
    a. Cover with 0.9% saline solution, then make ventral incisions.
    b. Pull the two cut sides apart and grab the bursa, then gently pull out until mucous glands and seminal vesicles come with it.
    c. Cut seminal vesicles away from mucous glands.
  3. Place seminal vesicle into a 50 ml beaker containing 1 ml sperm diluent (see Table 4 for recipe).