5.5.2. Equipment

  • Fluorescent microscope with Rhodamine and FITC filters (for live and dead cells, respectively)
  • Dissecting microscope
  • Haemocytometer
  • Micro-weight balance
  • Micro-calipers
  • Microdispenser (e.g. Drummond, Fisher Scientific #3-000-225)
  • 2 ml glass screw-thread vials and caps (Fisher Scientific #03-391-16)
  • Two forceps, dissection tray, and pins
  • 1.5 ml microcentrifuge tubes (if you want to save the rest of the queen)
  • Water bath set at 36°C