2.1. Important experimental design considerations before caging adult workers in the laboratory
Although this paper discusses maintenance of adult worker honey bees in a laboratory outside of a colony, regardless of study type or purpose, it is important to highlight that careful consideration needs to be given to experimental design and statistical analysis of the ensuing data before any practical work should commence. Importantly, one must determine if sufficient resources are available to perform rigorous research with an appropriate level of reproducibility; if constraints preclude good science, it may not be worth conducting experiments in the first place.
General recommendations for design of experiments and analysis of data can be found in the BEEBOOK paper on statistical methods (Pirk et al., 2013).