4.3.2. Challenges associated with collecting adult workers of an undefined age for laboratory experiments

Obtaining workers of an undefined age for an experiment usually requires the collector to physically open the colony or stand immediately in front of it to retrieve individuals. Collecting flying workers at the colony entrance can particularly agitate colonies, and may initiate a defensive response that will result in a mass exodus of guards from the hive (Breed et al., 2004). Thus, agitation of colonies should be minimized because it can influence worker collection.