Collecting flying adult workers of an undefined age for laboratory experiments using a forceps

Exiting workers can be collected individually using forceps.

  1. Stand beside, and not in front of, the colony.
  2. During normal flight activity, grasp appropriate individuals by a leg or wing using forceps. Care must be taken that individuals are not damaged during collection. Refer to Human et al. (2013) in the miscellaneous methods paper of the BEEBOOK for details on handling honey bees using forceps.
  3. Place collected workers in a ventilated hoarding cage with appropriate food (see sections 5 and 7).
  4. Immediately transfer the hoarding cage to a laboratory incubator maintained at conditions discussed in section 6.