5.3.3. Examples of isolation cages in which to maintain adult workers in the laboratory
For isolation cages, modified straws with pins placed
at either end, 1.5-ml microcentrifuge tubes with breathing holes drilled
through the tip (Fig. 17), or 0.8-cm wide plastic Eppendorf tubes cut in half
longitudinally with sticky tape restraining harnesses (Fig. 18), can be used. To
our knowledge, researchers do not maintain individuals in these types of cages
for more than one week. Future studies should investigate effects of isolation cages
on survival and health of caged honey bees, as well as work to develop an
appropriate method for maintaining individuals in isolation cages for an
extended period of time. This could potentially greatly increase experiment
sample size compared to hoarding cages that must include cages, rather than individuals,
as number of replicates.
Fig. 18. Isolation cage constructed using a plastic Eppendorf tube cut in half longitudinally and sticky tape harnesses. Tube height, and outer and inner diameters = 3, 1, and 0.8 cm, respectively. Cages courtesy of CWW Pirk. Photo courtesy of V. Dietemann.