7.3.4. Replenishing proteins provided to caged adult workers in the laboratory

Similar to carbohydrates, care must be taken when replenishing proteins to avoid harming caged workers. Feeding pre-trials should be performed to determine quantity needed to ensure workers are fed ad libitum. Daily worker consumption should not exceed 3 mg protein; therefore, 3 g of protein paste, at least made from corbicular pollen pellets, should be sufficient to meet daily needs of 100 caged workers. Protein should be replaced at least every three days to prevent drying and microbial growth.