2.2.2. Weighing of larvae

  1. Carefully take the larva out of its cell.
    Injuries of larval surfaces result in the loss of haemolymph and injured larvae must be discarded.
    If the larvae are to be weighed when stored frozen, they have to be brought to room temperature to prevent convection during weighing and then weighed quickly before desiccation.
  2. 2. Wash larvae with either saline, alcohol or distilled water to remove remainders of the    larval food.
  3. Quickly dry them on filter paper.
    Due to the low weight and quick drying up of young larvae, time between sampling and weighing should be minimized.
  4. Tare the balance with container.
  5. Place larvae in container.
  6. Weigh larvae.
  7. Record weight.