Procedure to obtain queen brood of known age

Follow steps 1 to 8 as described in section

9. Allow for larval hatching approximately 3 days later.

10. Graft larvae of similar age, but younger than 24h into queen cups.

11. Follow the instructions given in the BEEBOOK paper on queen rearing and selection for queen rearing (Büchler et al., 2013).

12. Collect queen brood when it reaches the desired age or observe developmental stages at regular intervals, according to the purpose of the experiment.