11.4.1. Fragmentation of DNA
- Fragment 5µg of high
molecular weight DNA using the Covaris S2 AFA System in a total volume of
Duty cycle 10%,
Number of cycles: 3,
This results in a total fragmentation-time of 180s.
- Confirm fragmentation with a 2100 Bioanalyzer (Agilent Technologies) using a DNA1000 chip, aiming for fragment sizes of 140bp on average for both queen and worker DNAs.