11.4.2. End-repair of sheared DNA


Having a blunt (neither strand longer than the other) end to each double-stranded DNA section is needed to attach the below adaptors (‘handles’ that help connect DNA during library formation), and this is achieved as follows:

  1. Concentrate fragmented DNA to a final volume of 75µl using a DNA Speed Vac.
  2. End-repair fragmented DNA in a total volume of 100µl using the Paired End DNA Sample Prep Kit (Illumina, PE-102-1001) following manufacturer’s protocol.