11.6.2. Bisulfite DNA conversion

  1. The Qiagen EpiTect Bisulfite Kit and the manufacture’s protocol is widely accepted as the most efficient and reliable kit for DNA conversion.  The amount of starting materials can range from 0.1 to 2 μg.
  2. Because DNA conversion with bisulfite is only ~98% efficient it is highly recommendable to repeat this protocol twice. 
  3. 1/10th of the second conversion reaction is sufficient for subsequent amplification.