11.7. RNA extraction

  1. RNA for analysis can be extracted using the TRIzol®/QIAGEN RNeasy combination method followed by the QIAGEN Mini or Rneasy Minelute Cleanup kit, or by the detailed protocol in the RNA methods section 4.3. above.
  2. RNA concentrations are then evaluated via Nanodrop (section 3.2.1) analyses and integrity assessed by gel electrophoresis (see section 4.4.).