2.2.2. Blender

An excellent, cheap alternative to the beadmills, especially for large volumes is homogenisation with a blender. 

  •  Pros: Large volume; rapid; uniform.
  •  Cons: Cross-contamination risk due to re-use of blender; incompatible with organic solvents; corrosion of blender due to salts.

  1. Add between 30-200 frozen bees to blender.
  2. Add 500 μl ice-cold buffer per bee.
  3. Homogenise by gradually raising the blender settings, for about 5 minutes total homogenization.