2.2.7. Robotic extraction

Companies produce robotic extraction stations to facilitate high-throughput analysis of samples. Comparisons between several such systems, or between automated and manual extraction, generally find little difference in terms of assay sensitivity and reproducibility (Rimmer et al., 2012; Bruun-Rasmussen et al., 2009; Agüero et al., 2007; Petrich et al., 2006; Knepp et al., 2003, but see Schuurman et al., 2005). Such systems are generally only suitable for easily disrupted, soft tissues or samples.

  • Pros: Single bees; rapid; high throughput; uniform; low cross contamination risk.
  • Cons: Expensive; inflexible protocols; soft tissues only.

Follow manufacturers’ protocol for sample processing.