9.3. Fixation of cultured cells grown in suspension

  1.  Transfer 100 µl of cultured cells into a cell funnel-chamber of a cell spin (Tharmac).
  2. Centrifuge the cells with 600 rpm (54xg) gently for 5 minutes on a glass slide (VWR).
  3. Remove the cell funnel-chamber.
  4. Let the medium air dry.
  5. Fix the cells in 4% formalin (Roth) at 4°C for 24 hours.