9.3. Fixation of cultured cells grown in suspension
- Transfer 100 µl of cultured cells into a cell funnel-chamber of a cell spin (Tharmac).
- Centrifuge the cells with 600 rpm (54xg) gently for 5 minutes on a glass slide (VWR).
- Remove the cell funnel-chamber.
- Let the medium air dry.
- Fix the cells in 4% formalin
(Roth) at 4°C for 24 hours.