2.2.3. Enzyme mix solution
The enzyme mix solution contains 1.5 mM NAD+, 1.0 mM ATP, 1.0 unit/ml of hexokinase, 1.0 unit/ml of glucose-6-phosphate dehydrogenase.
This enzyme mix is commercially available (Sigma-Aldrich) and dissolves readily in ddH2O. When kept at 4ºC, this solution should be stable for up to one month.