3.1.2. Glassware preparation

JH is a “sticky” lipophilic molecule which makes the use of clean glassware an imperative component throughout all steps of sample preparation. Furthermore, organic solvents can extract compounds from plastic vials, thus preventing accurate assessment of JH, especially when using a GC-MS protocol.  Thus:

  • Do not use plastic vials at any step and use disposable glassware whenever possible.
  • Do not mix glassware used in the preparation of stock solutions from commercial JH with those used in the preparation of biological samples.
  • It is strongly recommended that glassware be extensively cleaned and heated to remove contaminants prior to use, especially if the glassware is being reused.

The following procedures for treating glassware are recommended. For JH-RIA