3.4.5. User Safety

The use of radioactive compounds ([23,24-3H(N)]ecdysone)  requires authorization for individual user and laboratory according to national regulations and guidelines. Working with gloves is required to avoid direct contact with skin, but no further protection from radiation is necessary due to the low energy of tritiated compounds. Disposable material (glass) used during handling of radioactive compounds and liquid residues, as well as scintillation cocktail containing radioactivity must be properly disposed of following national guidelines. Dichloromethane and methanol are solvents harmful to health if used improperly. UV-light (254 nm) causes DNA damage and is especially harmful to the eyes; use protective glasses or a UV shield.