Fig. 9. Example of a measurement chamber for use in a temperature controlled water bath. It was milled out of a brass block. Air inlet visible in the picture bottom (front), outlet on top (covered by yellow kitchen cloth). Effective chamber volume is variable by a movable, perforated brass barrier. In this setup, the window in the lid was covered by an infrared transmissive plastic film allowing visual and thermographic behavioural observations and body surface temperature measurements (Kovac et al., 2007; Stabentheiner et al., 2012). Lid tightness was assured by a Viton® O-ring (not visible). Service holes were drilled in one side (left) to accept chromatography septa (11 mm), which allowed tight insertion of thermocouple wires. Tightness has to be proofed by applying an overpressure on the submerged chamber. The rectangle at the right-hand side is a proprietary reference radiator for infrared camera calibration (see Stabentheiner et al., 2012). Photo by Anton Stabentheiner.