Number of pollen traps

Most studies will require data to be collected from a number of colonies as there can be large differences in the plant species neighbouring colonies are visiting. The level of replication required will depend on the amount of variation between colonies and the size of the difference to be detected. Balancing colonies with regard to the amount of brood and number of bees they contain (see Delaplane et al., 2013) will reduce the amount of variation in the total amount of the pollen collected.