6. References

ALEXANDER, D H; NOVEMBRE, J; LANGE, K (2009) Fast model-based estimation of ancestry in unrelated individuals. Genome Research 19: 1655-1664. http://dx.doi.org/10.1101/gr.094052.109.

ALPATOV, W W (1929) Biometrical studies on variation and races of the honey bee (Apis mellifera L.). Quarterly Review of Biology 4: 1–58.

ALBURAKI, M; MOULIN, S; LEGOUT, H; ALBURAKI, A; GARNERY, L (2011) Mitochondrial structure of Eastern honey bee populations from Syria, Lebanon and Iraq. Apidologie 42: 628-641.http://dx.doi.org/10.1007/s13592-011-0062-4.

ANDERSON, E C; THOMPSON, E A (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160: 1217-1229.

ARIAS, M C; SHEPPARD, W S (1996) Molecular phylogenetics of honey bee subspecies (Apis mellifera) inferred from mitochondrial DNA sequence. Molecular Phylogenetics and Evolution 5: 557–566. http://dx.doi.org/DOI:10.1006/mpev.1996.0050.

ARIAS, M C; RINDERER, T E; SHEPPARD, W S (2006) Further characterization of honey bees from the Iberian Peninsula by allozyme, morphometric and mtDNA haplotype analyses. Journal of Apicultural Research  45: 188– 196. http://dx.doi.org/10.3896/IBRA.

BADINO, G; CELEBRNO, G; MANINO, A (1983) Population structure and Mdh-1 locus variation in Apis mellifera ligustica. Journal of Heredity 74: 443–446.

BADINO, G; CELEBRANO, G; MANINO, A; LONGO, S (1985) Enzyme polymorphism in the Sicilian honey bee. Experientia  41: 752–754. http://dx.doi.org/10.1007/BF02012580.

BADINO, G; CELEBRANO, G; MANINO, A; IFANTIDIS, M D (1988) Allozyme variability in Greek honey bees (Apis mellifera L). Apidologie 19: 337-386. http://dx.doi.org/10.1051/apido:19880405.

BAYLAC, M; GARNERY, L; THARAVY, D; PEDRAZA-ACOSTA, J; RORTAIS, A; ARNOLD, G (2008) ApiClass, an automaticwing morphometric expert system for honey bee identification, [online] http://apiclass.mnhn.fr.

BODUR, C; KENCE, M; KENCE, A (2007) Genetic structure of honey bee, Apis mellifera L. (Hymenoptera: Apidae) populations of Turkey inferred from microsatellite analysis. Journal of Apicultural Research  46(1): 50–56. http://dx.doi.org/10.3896/IBRA.

BOOKSTEIN, F L (1991) Morphometric tools for landmark data. Geometry and biology. Cambridge University Press; New York, USA. ISBN 0-521-38385.

BOUGA, M; KILIAS, G; HARIZANIS, P C; PAPASOTIROPOULOS, V; ALAHIOTIS, S (2005a) Allozyme variability and phylogenetic relationships in honey bee (Hymenoptera: Apidae: A. mellifera) populations from Greece and Cyprus. Biochemical Genetics 43: 471-484. http://dx.doi.org/10.1007/s10528-005-8163-2.

BOUGA, M; HARIZANIS, P C; KILIAS, G; ALAHIOTIS, S (2005b) Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR - RFLP analysis of three mtDNA Segments. Apidologie 36: 335-344. http://dx.doi.org/10.1051/apido:2005021.

BOUGA, M; ALAUX, C; BIENKOWSKA, M; BÜCHLER, R; CARRECK, N L; CAUIA, E; CHLEBO. R; DAHLE, B; DALLOLIO, R; DE LA RÚA. P; GREGORC, A; IVANOVA, E; KENCE, A; KENCE, M; KEZIC, N; KIPRIJANOVSKA, H; KOZMUS, P; KRYGER, P; LE CONTE, Y; LODESANI, M; MURILHAS, A M; SICEANU, A; SOLAND, G; UZUNOV, A; WILDE, J (2011): A review of methods for discrimination of honey bee populations as applied to European beekeeping. Journal of Apicultural Research 50: 51-84 http://dx.doi.org/10.3896/IBRA.

BOYER, S H (1961) Alkaline phosphatase in human sera and placentae. Science 134: 1002-1004. http://dx.doi.org/10.1126/science.134.3484.1002.

BRÜCKNER, D (1974) Reduction of biochemical polymorphism in honey bees (Apis mellifica). Experientia 30: 618-619. http://dx.doi.org/10.1007/BF01921504.

BRUMFIELD, R T; BEERLI, P ; NICKERSON, D A; EDWARDS, S V (2003) The utility of single nucleotide polymorphisms in inferences of population history. Trends in Ecology & Evolution 18: 249-256. http://dx.doi.org/10.1016/S0169-5347(03)00018-1.

CÁNOVAS, F; DE LA RÚA, P; SERRANO, J; GALIÁN, J (2008) Geographical patterns of mitochondrial DNA variation in Apis mellifera iberiensis (Hymenoptera: Apidae). Journal of Zoological Systematics and Evolutionary Research 46(1): 24–30. http://dx.doi.org/10.1111/j.1439-0469.2007.00435.x

CÁNOVAS, F; DE LA RÚA, P; SERRANO, J; GALIÁN, J (2011) Microsatellite variability reveals beekeeping influences on Iberian honey bee populations. Apidologie 42: 235-251. http://dx.doi.org/10.1007/s13592-011-0020-1.

CHAPUIS, M-P; ESTOUP, A (2007) Microsatellite null alleles and estimation of population differentiation. Molecular Biology and Evolution 24(3): 621-631. http://dx.doi.org/10.1093/molbev/msl191.

CLARKE, K E; RINDERER, T E; FRANCK, P; QUEZADA-EUÁN, J J G; OLDROYD, B P (2002) The Africanization of honey bees ( Apis mellifera L.) of the Yucatan : a study of a massive hybridization event across time. Evolution 56(7): 1462–1474. http://dx.doi.org/10.1111/j.0014-3820.2002.tb01458.x

COELHO, J R; MITTON, J B (1988) Oxygen consumption during hovering is associated with genetic variation of enzymes in honey bees. Functional Ecology 2: 141-146.

COLLET, T; FERREIRA, K M; ARIAS, M C; SOARES, A E E; DEL LAMA, M A (2006). Genetic structure of Africanized honey bee populations (Apis mellifera L.) from Brazil and Uruguay viewed through mitochondrial DNA COI–COII patterns. Heredity 97: 329–335. http://dx.doi.org/10.1038/sj.hdy.6800875.

CORNUET, J M (1982) The MDH polymorphism in some west Mediterranean honey bee populations. In Proceedings of the 9th Congress of the International Union for the Study of Social Insects, Boulder, Colorado, USA. pp. 5-6.

Cornuet, J M; Fresnaye, J (1979) Production de miel chez des hybrides interraciaux dabeilles (Apis mellifica L.) lors de générations successives de rétrocroisement sur la race locale. Apidologie 10: 3-15. http://dx.doi.org/10.1051/apido:19790101.

CORNUET, J M; FRESNAYE, J (1989) Etude biométrique de colonies d’abeilles d’Espagne et du Portugal. Apidologie 20(1): 93–101. http://dx.doi.org/10.1051/apido:19890109

CORNUET, J M; DAOUDI, A; CHEVALET, C (1986) Genetic pollution and number of matings in a black honey bee (Apis mellifera mellifera) population. Theoretical and Applied Genetics 73: 223-227. http://dx.doi.org/10.1007/BF00289278

CORNUET, J M; GARNERY, L; SOLIGNAC, M (1991) Putative origin and function of the intergenic region between COI and COII of Apis mellifera L. mitochondrial DNA. Genetics 128: 393-403.

CORNUET, J M; OLDROYD, B P; CROZIER, R H (1995) Unequal thermostability of allelic forms of malate dehydrogenase in honey bees. Journal of Apicultural Research 34: 45-47.

CORNUET, J M; PIRY, S; LUIKART, G; ESTOUP, A; SOLIGNAC, M (1999) New methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genetics 153: 1989-2000.

CROZIER, Y C; KOULIANOS, S; CROZIER, R H (1991). An improved test for Africanized honey bee mitochondrial DNA. Experientia  47: 968-969. http://dx.doi.org/10.1007/BF01929894.

CURRIE, R W; JAY, S C (1988) The influence of a colony’s queen state, time of the year, and drifting behaviour, on the acceptance and longevity of adult drone honey bees (Apis mellifera L.), Journal of Apicultural Research 27: 219–226.

DALL’OLIO, R; MARINO, A; LODESANI, M; MORITZ, R F A (2007) Genetic characterization of Italian honey bees, Apis mellifera ligustica, based on microsatellite DNA polymorphisms. Apidologie 38: 207–217. http://dx.doi.org/10.1051/apido:2006073.

DALY, H V; HOELMER, K; NORMAN, P; ALLEN, T (1982) Computer assisted measurement and identification of honey bees. Annals of the Entomological Society of America 75: 591–594.

DAVIS, B J (1964). Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121: 404 427. http://dx.doi.org/10.1111/j.1749-6632.1964.tb14213.x

DEDEJ, S; BASIOLO, A; PIVA, R (1996) Morphometric and alloenzymatic characterisation in the Albanian honey bee population Apis mellifera L. Apidologie 27(3): 121-131. http://dx.doi.org/10.1051/apido:19960301

DE LA RÚA, P; SERRANO, J; GALIÁN J (1998) Mitochondrial DNA variability in the Canary Islands honey bees (Apis mellifera L.). Molecular Ecology 7(11): 1543–1547. http://dx.doi.org/10.1046/j.1365-294x.1998.00468.x

DE LA RÚA, P; GALIÁN, J; SERRANO, J; MORITZ, R F A (2001) Genetic structure and distinctness of Apis mellifera L: populations from the Canary Islands. Molecular Ecology 19: 1733-1742. http://dx.doi.org/10.1046/j.1365-294X.2001.01303.x

DE LA RÚA, P; GALIÁN, J; SERRANO, J; MORITZ, R F A (2001) Molecular characterization and population structure of the honey bees from the Balearic Islands (Spain). Apidologie 32: 417-427. http://dx.doi.org/10.1051/apido:2001141

DE LA RÚA, P; SERRANO, J; GALIÁN J (2002)  Biodiversity of Apis mellifera populations from Tenerife (Canary Islands) and introgressive hybridization with East European races. Biodiversity and Conservation 11: 59-67. http://dx.doi.org/10.1023/A:1014066407307

DE LA RÚA, P; GALIÁN, J; SERRANO, J; MORITZ, R F A (2003) Genetic structure of Balearic honey bee populations based on microsatellite polymorphism. Genetics, Selection, Evolution 35: 339-350. http://dx.doi.org/10.1186/1297-9686-35-3-339

DE LA RÚA, P; HERNANDEZ-GARCIA, R; JIMÉNEZ, Y; GALIÁN, J; SERRANO, J (2005) Biodiversity of Apis mellifera iberica (Hymenoptera : Apidae) from northeastern Spain assessed by mitochondrial analysis. Insect Systematics and Evolution 36: 21-28.

DE LA RÚA, P; GALIÁN, J; PEDERSEN, B V; SERRANO, J (2006) Molecular characterization and population structure of Apis mellifera from Madeira and the Azores. Apidologie 37: 699-708. http://dx.doi.org/10.1051/apido:2006044

DE LA RÚA, P; JAFFÉ, R; DALLOLIO, R; MUÑOZ, I; SERRANO, J (2009) Biodiversity, conservation and current threats to European honey bees. Apidologie 40(3): 263-284. http://dx.doi.org/10.1051/apido/2009027

DEL LAMA, M A; MESTRINER, M A; PAVIA, J C A (1985) Ast-5 and Pgm-1: new polymorphism in Apis mellifera. Brazilian Journal of Genetics 8: 17-27.

DEL LAMA, M A; FIGUEIREDO, R A; SOARES, A E E; DEL LAMA, S N (1988) Hexokinase polymorphism in Apis mellifera and its use for Africanized honey bee identification. Brazilian Journal of Genetics 11: 287-292.

DEL LAMA, M A; LOBO, J A; SOARES, A E E; DEL LAMA, S N (1990) Genetic differentiation estimated by isozymic analysis of Africanized honey bee populations from Brazil and from Central America. Apidologie 21: 271-280. http://dx.doi.org/10.1051/apido:19900401.

DINIZ-FILHO, J A F; HEPBURN, H R; RADLOFF. S; FUCH, S (2000) Spatial analysis of morphological variation in African honey bees (Apis mellifera L.) on a continental scale. Apidologie 31: 191–204. http://dx.doi.org/10.1051/apido:2000116

DU PRAW, E J (1964) Non-Linnean taxonomy. Nature 202: 849-852.

DUPRAW, E J (1965) Non-Linnean taxonomy and the systematics of honey bees. Systematic Zoology 14: 1-24.

DURAND, E; JAY, F; GAGGIOTTI, O E; FRANÇOIS, O (2009) Spatial inference of admixture proportions and secondary contact zones. Molecular Biology and Evolution 26:1963-1973. http://dx.doi.org/10.1093/molbev/msp106.

ESTOUP, A; SOLIGNAC, M; HARRY, M; CORNUET, J M (1993) Characterization of (GT)n and (CT)n microsatellites in two insect species Apis mellifera and Bombus terrestris. Nucleic Acids Research 21: 1427-1431. http://dx.doi.org/10.1093/nar/21.6.1427.

Estoup, A; Garnery, L; solignac, M; Cornuet, J M (1995a) Microsatellite variation in honey bee (Apis mellifera L) populations: Hierarchical genetic structure and test of the infinite allele and stepwise mutation models. Genetics 140: 679-695.

ESTOUP, A; TAILLIEZ, C; CORNUET, J M; SOLIGNAC, M (1995b) Size homoplasy and mutational processes of interrupted microsatellites in two bee species, Apis mellifera and Bombus terrestris (Apidae). Molecular Biology and Evolution 12: 1074-1084.

EVANNO, G; REGNAUT, S; GOUDET, J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14(8) 2611-2620. http://dx.doi.org/10.1111/j.1365-294X.2005.02553.x

EVANS, J D; CHEN, Y P; CORNMAN, R S; DE LA RUA, P; FORET, S; FOSTER, L; GENERSCH, E; GISDER, S; JAROSCH, A; KUCHARSKI, R; LOPEZ, D; LUN, C M; MORITZ, R F A; MALESZKA, R; MUÑOZ, I; PINTO, M A; SCHWARZ, R S (2013) Standard methodologies for molecular research in Apis mellifera. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(4):http://dx.doi.org/10.3896/IBRA.

EXCOFFIER, L; LAVAL, G; SCHNEIDER, S (2005) Arlequin (version 30): An integrated software package for population genetics data analysis. Evolutionary Bioinformatics 1: 47-50.

FRANCK, P; GARNERY, L; SOLIGNAC, M; CORNUET, J M (1998) The origin of west European subspecies of honey bees (Apis mellifera): New insights from microsatellite and mitochondrial data. Evolution 52(4): 1119–1134. http://dx.doi.org/10.2307/2411242

FRANCK, P; GARNERY, L; CELEBRANO, G; SOLIGNAC, M; CORNUET, J M (2000) Hybrid origin of honey bees from Italy (Apis mellifera ligustica) and Sicily (A. m. sicula). Molecular Ecology 9: 907–921. http://dx.doi.org/10.1046/j.1365-294x.2000.00945.x

FRANCK, P; GARNERY, L; LOISEAU, A (2001) Genetic diversity of the honey bee in Africa: microsatellite and mitochondrial data. Heredity 86: 420–430. http://dx.doi.org/10.1046/j.1365-2540.2001.00842.x

FRANCK, P; GARNERY, L; SOLIGNAC, M; CORNUET, J M (2000) Molecular confirmation of a fourth lineage in honey bees from the Near East. Apidologie 31: 167-180. http://dx.doi.org/10.1051/apido:2000114

FRANCOY, T M; PRADO, P R R; GONÇALVES, L S; DA FONTOURA COSTA, L; DE JONG D (2006) Morphometric differences in a single wing cell can discriminate Apis mellifera racial types. Apidologie 37: 91-97. http://dx.doi.org/10.1051/apido:2005062

FRANCOY, T M; WITTMANN, D; DRAUSCHKE, S; MÜLLER. S; STEINHAGE, V (2008) Identification of Africanized honey bees through wing morphometrics: two fast and efficient procedures. Apidologie 39(5):488-494. http://dx.doi.org/10.1051/apido:2008028

GAHNE, B (1967) Alkaline phosphatase isoenzyme in serum and seminal plasma. Hereditas 57: 83-99. http://dx.doi.org/10.1111/j.1601-5223.1967.tb02094.x

GARNERY, L; CORNUET, J M; SOLIGNAC, M (1992) Evolutionary history of the honey bee  Apis mellifera inferred from mitochondrial DNA analysis. Molecular Ecology 1: 145-154. http://dx.doi.org/10.1111/j.1365-294X.1992.tb00170.x

GARNERY, L; SOLIGNAC, M; CELEBRANO, G; CORNUET, J M (1993) A simple test using restricted PCR-amplified mitochondrial-DNA to study the genetic-structure of Apis mellifera L. Experientia 49(11): 1016–1021. http://dx.doi.org/10.1007/BF02125651

GARNERY, L; FRANCK, P; BAUDRY, E; VAUTRIN, D; CORNUET, J M; SOLIGNAC, M (1998a) Genetic diversity of the west European honey bee (Apis mellifera and A. m. iberica). I. Mitochondrial DNA. Genetics Selection Evolution 30:S 31-47. http://dx.doi.org/10.1186/1297-9686-30-S1-S31

GARNERY, L; FRANCK, P; BAUDRY, E; VAUTRIN, D; CORNUET, J M; SOLIGNAC, M (1998b) Genetic biodiversity of the West European honey bee (Apis mellifera mellifera and Apis mellifera iberica). II. Microsatellite loci. Genetics Selection Evolution 30: 49– S74. http://dx.doi.org/10.1051/gse:19980703

GARSTIDE, D F (1980) Similar allozyme polymorphism in honey bees (Apis mellifera) from different continents. Experientia 36: 649-650.

GOUDET, J (1995) FSTAT (Version 12): A computer program to calculate F-statistics Journal of Heredity 86: 485-486.

GOETZE, G (1964) Die Honigbiene in natürlicher und künstlicher Zuchtauslese Parey, Hamburg.

GUILLOT, G; MORTIER, F; ESTOUP, A (2005) Geneland: A program for landscape genetics. Molecular Ecology Notes 5: 712-715. http://dx.doi.org/10.1111%2Fj.1471-8286.2005.01031.x

HALL, H G; MURALIDHARAN, K (1989) Evidence from mitochondrial DNA that African honey bees spread as continuous maternal lineages. Nature 339: 211-213. http://dx.doi.org/10.1038/339211a0

HALL, H G; SMITH, D R (1991) Distinguishing African and European honey bee matrilines using amplified mitochondrial DNA. Proceedings of the National Academy of Sciences 88: 4548-4552. http://dx.doi.org/10.1073/pnas.88.10.4548

HARRIS, H; HOPKINSON, D A (1976) Handbook of enzyme electrophoresis in human genetics. North-Holland Publishing Company; Amsterdam, Netherlands.

HEPBURN, H R; RADLOF, S E (1996) Morphometric and pheromonal analysis of Apis mellifera L. along a transect from the Sahara to the Pyrenees. Apidologie 27: 35–45. http://dx.doi.org/10.1051/apido:19960105

HEPBURN, H R; RADLOFF, S E (1998) Honey bees of Africa. Springer; Berlin, Germany.

HUMAN, H; BRODSCHNEIDER, R; DIETEMANN, V; DIVELY, G; ELLIS, J; FORSGREN, E; FRIES, I; HATJINA, F; HU, F-L; JAFFÉ, R; KÖHLER, A; PIRK, C W W; ROSE, R; STRAUSS, U; TANNER, G; TARPY, D R; VAN DER STEEN, J J M; VEJSNÆS, F; WILLIAMS, G R; ZHENG, H-Q (2013) Miscellaneous standard methods for Apis mellifera research. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(4): http://dx.doi.org/10.3896/IBRA.

IVANOVA, E (2010) Investigation on genetic variability in honey bee populations from Bulgaria, Greece and Serbia. Biotechnology & Biotechnological Equipment 24(2): 385–389.

IVANOVA, E; STAYKOVA, T; BOUGA, M (2007) Allozyme variability in honey bee populations from some mountainous regions in southwest of Bulgaria. Journal of Apicultural Research 46(1): 3-7. http://dx.doi.org/10.3896/IBRA.

IVANOVA, E; PETROV, P; BOUGA, M; EMMANOUEL, N; IVGIN-TUNKA, R; KENCE, M (2010a) Genetic variation in honey bee (Apis mellifera L.) populations from Bulgaria. Journal of Apicultural Science 54(2): 49-60.

IVANOVA, E; STAYKOVA, T; PETROV, P (2010b). Allozyme variability in populations of local Bulgarian honey bee. Biotechnology & Biotechnological Equipment 24(2): 371–374.

IVANOVA, E; BOUGA, M; PETROV, P; MLADENOVIC, M; RASIC, S; CHARISTOS, L; HATJINA, F (2010c) Preliminary results from a study on Balkan honey bees’ genetic variability using isoenzymic approach. In Proceedings of 4th European Conference of Apidology, 7-9 September 2010, Ankara, Turkey. p 45.

IVANOVA, E; BIENKOWSKA, M; PETROV, P (2011) Allozyme polymorphism and phylogenetic relationships in Apis mellifera subspecies selectively reared in Poland and Bulgaria. Folia biologica 59: 3-4. http://dx.doi.org/10.3409/fb59_3-4.121-126

JAFFÈ, R; DIETEMANN, V; ALLSOPP, M H; COSTA, C; CREWE, R M; DALL’OLIO R; DE LA RÚA, P; EL-NIWEIRI, M A A; FRIES, I; KEZIC, N; MEUSEL M S; PAXTON R J; SHAIBI, T; STOLLE, E; MORITZ, R F A (2010) Estimating the density of honey bee colonies across their natural range to fill the gap in pollinator decline censuses. Conservation Biology 24: 583-593. http://dx.doi.org/10.1111/j.1523-1739.2009.01331.x

JAY, S C (1969) The problem of drifting in commercial apiaries. American Bee Journal 109: 178–179.

JENSEN, A B; PALMER, K A; BOOMSMA, J J; PEDERSEN, B V (2005) Varying degrees of Apis mellifera ligustica introgression in protected populations of the black honey bee, Apis mellifera mellifera, in northwest Europe. Molecular Ecology 14: 93–106. http://dx.doi.org/10.1111/j.1365-294X.2004.02399.x

JOMBART, T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24: 1403-1405. http://dx.doi.org/10.1093/bioinformatics/btn129

JONES, J C; MYERSCOUGH, M R; GRAHAM, S; OLDROYD, B P (2004) Honey bee nest thermoregulation: diversity promotes stability. Science 305: 402-404. http://dx.doi.org/10.1126/science.1096340

KANDEMIR, I; KENCE, A (1995) Allozyme variation in a central Anatolian honey bee (Apis mellifera L.) population. Apidologie 26: 503-510. http://dx.doi.org/10.1051/apido:19950607

KANDEMIR, I; KENCE, M; KENCE, A (2000) Genetic and morphometric variation in honey bee (Apis mellifera L.) populations of Turkey. Apidologie 31: 343-356. http://dx.doi.org/10.1051/apido:2000126

KANDEMIR, I; KENCE, M; KENCE, A (2005) Morphometric and electrophoretic variation in different honey bee (Apis mellifera L.) populations. Turkish Journal of Veterinary and Animal Science 29: 885-890.

KANDEMIR, I; KENCE, M; SHEPPARD W S; KENCE, A (2006) Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey. Journal of Apicultural Research  45(1): 33–38. http://dx.doi.org/10.3896/IBRA.

KANDEMIR, I; ÖZKAN, A; FUCHS, S (2011) Reevaluation of honey bee (Apis mellifera) microtaxonomy: a geometric morphometric approach. Apidologie 42: 618–627. http://dx.doi.org/10.1007/s13592-011-0063-3

KLINGENBERG, C P (2011) Morpho J: an integrated software package for geometric morphometrics. Molecular Ecology Resources 11: 353-357.

LEVINSON, G; GUTMAN, G A (1987) Slipped-Strand Mispairing: A major mechanism for DNA sequence evolution. Molecular Biology and Evolution 4: 203-221.

LOBO, J A; DEL LAMA, M A; MESTRINER, M A (1989) Population differentiation and racial admixture in the Africanized honey bee (Apis mellifera L). Evolution 43: 794-802. http://dx.doi.org/10.2307/2409307

Louveaux, J; Albisetti, M; Delangue, M; Theurkauff, J (1966) Les modalités de ladaptation des abeilles (Apis mellifica L.) au milieu naturel. Annales de l Abeille 9: 323-350. http://dx.doi.org/10.1051/apido:19660402

MANEL, S; GAGGIOTTI, O E; WAPLES, R E (2005) Assignment methods: matching biological questions with appropiate techniques. Trends in Ecology and Evolution 20(3): 136-142. http://dx.doi.org/10.1016/j.tree.2004.12.004

MĂRGHITAS, L A; PANITI-TELEKY, O; DEZMIREAN, D; MĂRGĂOAN, R, BOJAN, C, COROIAN, C, LASLO, L; MOISE, A (2008) Morphometric differences between honey bee (Apis mellifera carpatica) populations from transyslvanian area. Lucrări stiińifice Zootehnie si Biotehnologii 41(2): Timisoara.

MARTIMIANAKIS, S; KLOSSA-KILIA, E; BOUGA, M; KILIAS, G (2011) Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5). Journal of Apicultural Research 50(1): 42-50. http://dx.doi.org/10.3896/IBRA.

MEIXNER, M; SHEPPARD, W S; POKLUKAR, J (1993) Asymmetrical distribution of a mitochondrial DNA polymorphism between two introgressing honey bee races. Apidologie 24: 147–153. http://dx.doi.org/10.1051/apido:19930207

MEIXNER, M D; SHEPPARD, W S; DIETZ, A; KRELL, R (1994) Morphological and allozyme variability in honey bees from Kenya. Apidologie 25: 188-202. http://dx.doi.org/10.1051/apido:19940207

MEIXNER, M D; WOROBIK, M; WILDE , J; FUCHS, S; KOENIGER, N (2007) Apis mellifera mellifera in eastern Europe - morphometric variation and determination of range limits. Apidologie 38: 191-197. http://dx.doi.org/10.1051/apido:2006068

MEIXNER, M D; COSTA, C; KRYGER, P; HATJINA, F; BOUGA, M; IVANOVA, E; BÜCHLER, R (2010) Conserving diversity and vitality for honey bee breeding. Journal of Apicultural Research 49(1): 85-92. http://dx.doi.org/10.3896/IBRA.

MEIXNER, M D; LETA, M A; KOENIGER, N; FUCHS, S (2011) The honey bees of Ethiopia represent a new subspecies of Apis mellifera – Apis mellifera simensis n. ssp.  Apidologie 42: 425-437. http://dx.doi.org/10.1007/s13592-011-0007-y

MENDES, M F M; FRANCOY, T M; NUNES-SILVA, P; MENEZES, C; IMPERATRIZ-FONSECA, V L (2007) Intra-populational variability of Nannotrigona testaceicornis Lepeletier 1836 (Hymenoptera, Meliponini) using relative warp analysis. Bioscience Journal 23: 147-152.

MESTRINER, M A (1969) Biochemical polymorphism in bees (Apis m. ligustica). Nature 223: 188-189.

MESTRINER, M A; CONTEL, E P B (1972) The P-3 and Est loci in the honey bee Apis mellifera. Genetics 72: 733-738.

MIGUEL, I; IRIONDO, M; GARNERY, L; SHEPPARDW S; ESTONBA, A (2007) Gene flow within the M evolutionary lineage of Apis mellifera: role of the Pyrenees, isolation by distance and post-glacial re-colonization routes in the western Europe. Apidologie 38: 141-155. http://dx.doi.org/10.1051/apido:2007007

MIGUEL, I; BAYLAC, M; IRIONDO, M; MANZANO, C; GARNERY, L; ESTONBA, A (2010) Both geometric morphometric and microsatellite data consistently support the differentiation of the Apis mellifera M evolutionary branch. Apidologie 42: 150–161. http://dx.doi.org/10.1051/apido/2010048

MONTEIRO, L R (1999) Multivariate regression models and geometric morphometrics: the search for causal factors in the analysis of shape. Systematic Biology 48: 192–199. http://dx.doi.org/10.1080/106351599260526

MLADENOVIĆ, M; RADOŠ, R; STANISAVLJEVIĆ, L Ž; RAŠIĆ S (2011) Morphometric traits of the yellow honey bee (Apis mellifera carnica) from Vojvodina (Northern Serbia) Archives of Biological Sciences, Belgrade 63(1): 251-257. http://dx.doi.org/10.2298/ABS1101251M

MUÑOZ, I; DALL´OLIO, R; LODESANI, M; DE LA RÚA, P (2008) Sequence variation in the mitochondrial tRNAleu-cox2 intergenic region of African and African-derived honey bee populations. In Proceedings of the 3rd European Conference of Apidology, Belfast, United Kingdom, 8-11 September 2008. p 73.

MUÑOZ, I; DALLOLIO, R; LODESANI, M; DE LA RÚA, P (2009) Population genetic structure of coastal Croatian honey bees (Apis mellifera carnica). Apidologie 40: 617-626. http://dx.doi.org/10.1051/apido/2009041

NAZZI, F (1992) Morphometric analysis of honey bees from an area of racial hybridization in northeastern Italy. Apidologie 23: 89–96. http://dx.doi.org/10.1051/apido:19920201

NEUMANN, P; MORITZ, R F A; MAUTZ, D (2000) Colony evaluation is not affected by drifting of drone and worker honey bees (Apis mellifera L.) at a performance testing apiary. Apidologie 31: 67-79. http://dx.doi.org/10.1051/apido:2000107

NIELSEN, D; PAGE, J R E; CROSLAND, M W J (1994) Clinal variation and selection of MDH allozymes in honey bee populations. Experientia 50: 867-871. http://dx.doi.org/10.1007/BF01956474

NIELSEN, D J; EBERT,  P R; PAGE, R E; HUNT, G J; GUZMAN-NOVOA, E (2000) Improved polymerase chain reaction-based mitochondrial genotype assay for identification of the Africanized honey bee (Hymenoptera:  Apidae). Annals of Entomological Society of America 93: 1-6. http://dx.doi.org/10.1603/0013-8746(2000)093[0001:IPCRBM]2.0.CO;2

NIKOLOVA, S (2011) Genetic variability of local Bulgarian honey bees Apis mellifera macedonica (rodopica) based on microsatellite DNA analysis. Journal of Apicultural Science 55(2): 117-129.

NEDIĆ N;  STANISAVLJEVIĆ L; MLADENOVIĆ M; JELENA STANISAVLJEVIĆ J (2009) Molecular characterization of the honey bee Apis mellifera carnica in Serbia. Archives of Biological Sciences, Belgrade 61(4): 587-598. http://dx.doi.org/10.2298/ABS0904587N

NUNAMAKER, R A; WILSON, W T; HALEY, B E (1984) Electrophoretic detection of Africanized honey bees (Apis mellifera scutellata) in Guatemala and Mexico based on malate dehydrogenase allozyme patterns. Journal of the Kansas Entomological Society 57: 622-631.

OREILLY, P T; HAMILTON, L C; McCONNELL, S K; WRIGHT, J M (1996) Rapid analysis of genetic variation in Atlantic salmon (Salmo salar) by PCR9 multiplexing of dinucleotide and tetranucleotide microsatellites. Canadian Journal of Fisheries and Aquatic Sciences 53: 2292-2298.

PALMER, M R; SMITH D R; KAFTANOĞLU,O (2000) Turkish honey bees: genetic variation and evidence for a fourth lineage of Apis mellifera mtDNA. Journal of Heredity 91: 42-66. http://dx.doi.org/10.1093/jhered/91.1.42

PADILLA ÁLVAREZ, F; HERNÁNDEZ  FERNÁNDEZ, R; REYES LÓPEZ, R; PUERTA, F; FLORES SERRANO, J M; BUSTOS RUIZ, M (1998) Estudio morfológico de las abejas melíferas del archipelago Canario (Gran Canaria, Tenerife, la Palma, Gomera) [Morphomological study of honey bees on the Canary Islands (Gran Canaria, Tenerife, la Palma, Gomera]. Archivos de Zootecnia 178: 451-459.

PADILLA ÁLVAREZ, F;  VALERIO DA SILVA, M J; CAMPANO CABANES, F; JIMÉNEZ VAQUERO, E; PUERTA PUERTA, F; FLORES SERRANO, J M; BUSTOS RUIZ, M (2001) Biometric study of Apis mellifera populations from central Portugal and Madeira. Archivos de Zootecnia 50(189): 67-77.  

PEAKALL, R; SMOUSE, P E (2006) GENALEX 6: genetic analysis in Excel Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295. http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x

PINTO, M.A; JOHNSTON, J S; RUBINK, W L; COULSON, R N; PATTON, J C; SHEPPARD, W S (2003) Identification of Africanized honey bee (Hymenoptera: Apidae) mitochondrial DNA: validation of a rapid PCR-based assay. Annals of the Entomological Society of America 96: 679-684. http://dx.doi.org/10.1603/0013-8746(2003)096[0679:IOAHBH]2.0.CO;2

PINTO, M A; RUBINK, W L; COULSON, R N; PATTON, J C; JOHNSTON, J S (2004) Temporal pattern of Africanization in a feral honey bee population from Texas inferred from mitochondrial DNA. Evolution 58: 1047–1055. http://dx.doi.org/10.1554/03-334

PINTO, M A; MUÑOZ, I; CHÁVEZ-GALARZA, J; DE LA RUA, P (2012) The Atlantic side of the Iberian peninsula: a hot-spot of novel african honey bee maternal diversity. Apidologie 43: 663-673.


PIRY, S; ALAPETITE, A; CORNUET, J. M; PAETKAU, D; BAUDOUIN, L; ESTOUP, A (2004) GeneClass2: a software for genetic assignment and first-generation migrant detection. Journal of Heredity 95: 536-539. http://dx.doi.org/10.1093/jhered/esh074

PRITCHARD, J K; STEPHENS, M; DONNELLY, P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945–959.

QUEZADA-EUÁN, J G; PÉREZ-CASTRO, E E; MAY-ITZÁ, W J (2003) Hybridization between European and African-derived honey bee populations (Apis mellifera) at different altitudes in Peru. Apidologie 34: 217-225. http://dx.doi.org/10.1051/apido:2003010

RADLOFF, S E; HEPBURN, H R (1997a) Multivariate analysis of honey bees, Apis mellifera L. (Hymenoptera: Apidae) on the horn of Africa. African Entomology 5: 57–64.

RADLOFF, S E; HEPBURN, H R (1997b) Multivariate analysis of honey bees, Apis mellifera Linnaeus (Hymenoptera: Apidae) from western central Africa. African Entomology 5: 195–204.

RADLOFF, S E; HEPBURN, H R (1998) The matter of sampling distance and confidence levels in the subspecies classification of honey bees, Apis mellifera L. Apidologie 29: 491-591. http://dx.doi.org/10.1051/apido:19980602

RADLOFF, S E; HEPBURN, H R; HEPBURN, C; DE LA RÚA, P (2001) Morphometric affinities and population structure of honey bees of the Balearic Islands in the Mediterranean Sea. Journal of Apicultural Research 40: 97-103.

RADLOFF, S E; HEPBURN, H R; LINDSEY J B (2003) Quantitative analysis of intracolonial and intercolonial morphometric variance in honey bees, Apis mellifera and Apis cerana. Apidologie 34: 339-351. http://dx.doi.org/10.1051/apido:2003034

RADLOFF, S E; HEPBURN, C; HEPBURN, H R; HADISOESILO, S; FUCHS, S; TAN, K; ENGEL, M S; KUZNETSOV, V (2010) Population structure and classification of Apis cerana. Apidologie 41: 589–601. http://dx.doi.org/10.1051/apido/2010008

RAYMOND, M; ROUSSET, F (1995) Genepop (version 12) - Population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248-249.

RÉKA, T O; BOJAN, C; MOISE, A; COROIAN, C; DEZMIREAN, D; MĂRGHITAS, L A (2007) Ecotypes differentiation within honey bees (Apis mellifera carpatica) from Transylvania. Bulletin USAMV-CN, 63-64.

RINDERER, T E; SYLVESTER H A; BROWN, M A; VILLA, J D; PESANTE, D; COLLINS, A M (1986) Field and simple techniques for identifying Africanized and European honey bees. Apidologie 17: 33-48. http://dx.doi.org/10.1051/apido:19860104

RINDERER, T E; SYLVESTER H A; BUCO, S M; LANCASTER, V A; HERBERT, E W; COLLINS, A M; HELLMICH, R L (1987) Improved simple techniques for Identifying Africanized and European honey bees. Apidologie 18: 179-196. http://dx.doi.org/10.1051/apido:19870208

ROHLF, F J (1990) NTSYS-pc, Numerical taxomomy and multivariate analysis system. Exeter Software; NY, USA.

ROHLF, F J (2000) Statistical power comparisons among alternative morphometric methods. American Journal of Physical Anthropology 111:463-478. http://dx.doi.org/10.1002/(SICI)1096-8644(200004)111:4<463::AID-AJPA3>3.0.CO;2-B

ROHLF, F J (2001) TPSdig: digitize landmarks from image files, scanner, or video. Department of Evolutionary Biology, University of New York; Stony Brook, NY, USA.

ROHLF, F J; SLICE, D. (1990) Extensions of the Procrustes method for the optimal superimposition of landmarks. Systematic Zoology 39: 40–59. http://dx.doi.org/10.2307/2992207

RORTAIS, A; ARNOLD G; ALBURAKI, M; LEGOUT, H; GARNERY, L (2011) Review of the DraI COI-COII test for the conservation of the black honey bee (Apis mellifera mellifera). Conservation Genetics Resources 3: 383-391. http://dx.doi.org/10.1007/s12686-010-9351-x

RUTTNER, F (1980) Apis mellifera adami (n.ssp.), die Kretische Biene. Apidologie 11: 385-400. http://dx.doi.org/10.1051/apido:19800407

RUTTNER, F (1988) Biogeography and taxonomy of honey bees. Springer-Verlag; Berlin, Germany. ISBN 0387177817.

RUTTNER, F; TASSENCOURT, L; LOUVEAUX, J (1978) Biometrical-statistical analysis of the geographic variability of Apis mellifera L. Apidologie 9: 363-381. http://dx.doi.org/10.1051/apido:19780408

SEELEY, T D; TARPY, D R (2007) Queen promiscuity lowers disease within honey bee colonies. Proceedings of the Royal Society B274: 67–72. http://dx.doi.org/10.1098/rspb.2006.3702

SHAIBI, T; MUNOZ, I; DALLOLIO, R; LODESANI, M; DE LA RUA, P; MORITZ, R F A (2009) Apis mellifera evolutionary lineages in Northern Africa: Libya, where orient meets occident. Insectes Sociaux  56: 293-300. http://dx.doi.org/10.1007/s00040-009-0023-3

SHAW, C R; PRASAD, R (1970) Starch-gel electrophoresis - a compilation of recipes. Biochemical Genetics 4: 297-320. http://dx.doi.org/10.1007/BF00485780

SHEPPARD, W S (1988) Comparative study of enzyme polymorphism in United States and European honey bee (Hymenoptera: Apidae) populations. Entomological Society of America 81: 886-889.

SHEPPARD, W S; BERLOCHER, S H (1984) Enzyme polymorphism in Apis mellifera from Norway. Journal of Apicultural Research 23: 64-69.

SHEPPARD, W S; BERLOCHER, S H (1985) New allozyme variability in Italian honey bees. Journal of Heredity 76: 45-48.

SHEPPARD, W S; MCPHERON, B A (1986) Genetic variation in honey bees from an area of racial hybridization in western Czechoslovakia. Apidologie 17: 21-32. http://dx.doi.org/10.1051/apido:19860103

SHEPPARD, W S; ARIAS, M C; GREECH, A; MEIXNER M D (1997) Apis mellifera ruttneri, a new honey bee subspecies from Malta. Apidologie 28: 287-293. http://dx.doi.org/10.1051/apido:19970505

SHEPPARD, W S; MEIXNER, M D (2003) Apis mellifera pomonella, a new honey bee subspecies from the Tien Shan mountains of Central Asia. Apidologie 34: 367-375. http://dx.doi.org/10.1051/apido:2003037

SCHNABEL, R D; WARD, T J; DERR J N (2000) Validation of 15 microsatellites for parentage testing in North American bison, Bison bison and domestic cattle. Animal Genetics 31: 360-366. http://dx.doi.org/10.1046/j.1365-2052.2000.00685.x

SINACORI, A; RINDERER, T E; LANCASTER, V; SHEPPARD, W S (1998) A morphological and mitochondrial assessment of Apis mellifera from Palermo, Italy. Apidologie 29: 481–490. http://dx.doi.org/10.1051/apido:19980601

SLICE, D E (2002) Morpheus, for morphometric research software. Department of Biomedical Engineering, Wake Forest University School of Medicine; Winston, Salem, USA.

SMITH, D R; BROWN, W M (1988) Polymorphisms in mitochondrial DNA of European and Africanized honey bees (Apis mellifera). Experientia 44: 257-260. http://dx.doi.org/10.1007/BF01941730

SMITH, D R; BROWN, W M (1990) Restriction endonuclease cleavage site and length polymorphisms in mitochondrial DNA of Apis mellifera mellifera and A. m. carnica (Hymenoptera: Apidae). Annals of the Entomological Society of America 83: 81-88.

SMITH, D R; GLENN, T (1995) Allozyme polymorphisms in Spanish honey bees.  Journal of Heredity 86: 12-16.

SMITH, D R; TAYLOR, O R; BROWN, W M (1989) Neotropical Africanized honey bees have African mitochondrial DNA. Nature 339: 213-215. http://dx.doi.org/10.1038/339213a0

SMITH, D R; SLAYMAKER, A; PALMER, M; KAFTANOGLU, O (1997) Turkish honey bees belong to the east Mediterranean mitochondrial lineage. Apidologie 28: 269-274. http://dx.doi.org/10.1051/apido:19970503

SMITH, D R; PALOPOLI, M F; TAYLOR, B R; GARNERY, L; CORNUET, J. M; SOLIGNAC, M; BROWN, W M (1991) Geographical overlap of two mitochondrial genomes in Spanish honey bees (Apis mellifera iberica). Journal of Heredity 82: 96-100.

SOLAND-RECKEWEG, G; HECKEL, G; NEUMANN, P; FLURI, P; EXCOFFIER, L (2009) Gene flow in admixed populations and implications for the conservation of the Western honey bee, Apis mellifera. Journal of Insect Conservation 13: 317-328. http://dx.doi.org/10.1007/s10841-008-9175-0

SOLIGNAC, M; VAUTRIN, D; LOISEAU, A; MOUGEL, F; BAUDRY, E; ESTOUP, A; GARNERY, L; HABERL, M; CORNUET, J M (2003) Five hundred and fifty microsatellite markers for the study of the honey bee (Apis mellifera L.) genome. Molecular Ecology Notes 3: 307-311. http://dx.doi.org/10.1046/j.1471-8286.2003.00436.x

SPÖTTER, A; GUPTA, P; NÜRNBERG, G; REINSCH, N; BIENEFELD, K (2012) Development of a 44K SNP assay focussing on the analysis of a varroa-specific defence behaviour in honey bees (Apis mellifera carnica). Molecular Ecology Resources 12: 323-332. http://dx.doi.org/10.1111/j.1755-0998.2011.03106.x

STEINHAGE, V; KASTENHOLZ, B; SCHRÖDER, S; DRESCHER, W (1997) A hierarchical approach to classify solitary bees based on image analysis. Mustererkennung 19. DAGM-Symposium, Braunschweig, Sept. 15–17, 1997, Informatik Aktuell; Springer, Germany. pp. 419–426.

STEINHAGE, V; SCHRÖDER, S; LAMPE, K H; CREMERS, A B (2007) Automated extraction and analysis of morphological features for species identification. In N MacLeod (Ed.). Automated object identification in systematics: theory, approaches, and applications. pp. 115–129

STEVANOVIC, J; STANIMIROVIC, Z; RADAKOVIC, M; KOVACEVIC, S R (2010) Biogeographic study of the honey bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia based on mitochondrial DNA analyses. Russian Journal of Genetics 46(5): 603–609. http://dx.doi.org/10.1134/S1022795410050145

STRANGE, J P; GARNERY, L; SHEPPARD W S (2007) Persistence of the Landes ecotype of Apis mellifera mellifera in southwest France: confirmation of a locally adaptive annual brood cycle trait. Apidologie 38: 259-267. http://dx.doi.org/10.1051/apido:2007012

SUŠNIK, S; KOZMUS, P; POKLUKAR, J; MEGLI, V (2004) Molecular characterisation of indigenous Apis mellifera carnica in Slovenia. Apidologie 35: 623–636. http://dx.doi.org/10.1051/apido:2004061

TARPY, D R (2003) Genetic diversity within honey bee colonies prevents severe infections and promotes colony growth. Proceedings of the Royal Society, London B270: 99-103. http://dx.doi.org/10.1098/rspb.2002.2199

TAUTZ, D (1990) Notes on the definition and nomenclature of tandemly repetitive DNA sequences. In S D J PENA; R CHAKRABORTY; J T EPPLEN; J JEFFREYS (Eds). DNA fingerprinting state of the science. Birkhauser; Basel, Switzerland. pp 21-28.

TOFILSKI, A (2004) DrawWing, a program for numerical description of insect wings. Journal of Insect Science 4: 1–5.

TOFILSKI, A (2008) Using geometric morphometrics and standard morphometry to discriminate three honey bee subspecies. Apidologie 39: 558-563. http://dx.doi.org/10.1051/apido:2008037

UZUNOV, A; KIPRIJANOVSKA, H; ANDONOV, S; NAUMOVSKI, M; GREGORC, A. (2009) Morphological diversity and racial determination of the honey bee (Apis mellifera L.) population in the Republic of Macedonia. Journal of Apicultural Research 48(3): 196-203. http://dx.doi.org/10.3896/IBRA.

UZUNOV, A; MEIXNER, M D; KIPRIJANOVSKA, H; ANDONOV, S; GREGORC, A; IVANOVA, E; BOUGA, M; DOBI, P; Francis, R M; BUECHLER, R; KRYGER, P (2014) Genetic structure of Apis mellifera macedonica population based on microsatellite DNA polymorphism. Journal of Apicultural Research (in press).

VIARD, F; FRANCK, P; DUBOIS, M-P; ESTOUP, A; JARNE, P (1998) Variation of microsatellite size homoplasy across electromorphs, loci and population in three invertebrate species. Journal of Molecular Evolution 47: 42-51 http://dx.doi.org/10.1007/PL00006361

VIGNAL, A; MILAN, D; SANCRISTOBAL, M; EGGEN, A (2002) A review on SNP and other types of molecular markers and their use in animal genetics. Genetics Selection Evolution 34: 275-305. http://dx.doi.org/10.1051/gse:2002009

Whitfield, C W; Behura, S K; Berlocher, S H; Clark, A G; Johnston, J S; Sheppard, W S; Smith, D R; Suarez, A V; Weaver, D; Tsutsui, N D (2006) Thrice out of Africa: ancient and recent expansions of the honey bee, Apis mellifera. Science 314: 642-645. http://dx.doi.org/10.1126/science.1132772

ZAYED, A; WHITFIELD, C W (2008) A genome-wide signature of positive selection in ancient and recent invasive expansions of the honey bee Apis mellifera. Proceedings of the National Academy of Sciences of the United States of America 105: 3421-3426. http://dx.doi.org/10.1073/pnas.0800107105

ZELDITCH, M L; SWIDERSKI, D L; SHEETS, H D; FINK, W L (2004) Geometric morphometrics for biologists: a primer. Elsevier Academic; New York, USA.