3.2.2. Topical application

The method outlined in this section (acute contact LD50) is based on the OECD guideline 214 (OECD, 1998b) to which later recommendations from EPPO Bulletin 40 (EPPO, 2010b) are added. This method was never ring-tested but was several times reviewed by OECD, EPPO and CEB. It is considered validated.