Preparation of doses

1) The test substance is to be applied as solution in acetone or as a water solution with a wetting agent. As an organic solvent, acetone is preferred but other organic solvents of low toxicity to bees may be used (e.g. dimethylformamide, dimethylsulfoxide). If others are used, they must be administered in the negative reference.

2) For water dispersed formulated products and highly polar organic substances not soluble in organic carrier solvents, solutions may be easier to apply if prepared in a solution of a commercial wetting agent to an extend the product dissolves (e.g. Agral, Citowett, Lubrol, Triton, and Tween).