3.2.2.3.2. Replicates

1) A minimum of three replicate test groups, each of ten bees, should be dosed with each test concentration. Bees in a single cage (a single replicate group) should be from the same colony, with a different colony being used to populate each cage.

2) The three replicates per dose of the PPP tested are treated with the same preparation of the test solution with a specific concentration, i.e. not a newly prepared test solution for each replicate group (Table 3).

Table 3. Test scheme for the acute contact LD50 test. “Conc.” = concentration.

Test solution

Replicate 1 (colony X)

Replicate 2 (colony Y)

Replicate 3 (colony Z)

Test conc.1

conc.1

conc.1

conc.1

Test conc. 2

conc. 2

conc. 2

conc. 2

Test conc. 3

conc. 3

conc. 3

conc. 3

Test conc. 4

conc. 4

conc. 4

conc. 4

Test conc. 5

conc. 5

conc. 5

conc. 5

Positive control conc. a

conc. A

conc. a

conc. a

Positive control conc. B

conc. B

conc. b

conc. b

Positive control conc. C

conc. C

conc. c

conc. c

Negative control [solvent: acetone (or other), water, or water with wetting agent]

Solvent

solvent

solvent

 

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