3.2.3.1.2.6. Dust application

1) 0.01 g of total dust (the AI-containing dust plus the talc powder) per cage should be distributed on the leaves (Table 4). This quantity was considered adequate for a homogeneous distribution on the surface of approximately 50-70 cm2. For bigger cages, a proportionally higher amount should be used.

2) A small sieve obtained from a modified Eppendorf tube can be used as shown in Fig. 1.

Fig. 1A. Small sieve obtained from a modified Eppendorf tube (the bottom is removed from the tube and replaced with screen mesh); B. dust application in the apple leaves; C. leaves placed in the bottom of the hoarding cage.

Figure 1

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