Duration of the test

Colonies remain on the edge of the field for the period of blossom. However, observations of the their evolution will be extended up to at least 42 days after the placement on the edge of the fields under study. This is the time of two complete brood cycles.

After blooming, they should be moved to an environment where they would overwinter together on the reserves they have accumulated during the trial period. The environment of the colonies should provide enough sources of pollen and nectar to survive. If necessary, colonies can be fed with syrup. This can be done making sure that the colony has consumed first its reserves collected during the exposure period. The colony should not be exposed to further contaminants contained in syrup. Residue analyses or tracking the syrup origin may help providing this information.

The colonies should be monitored through the following season. In the event that pesticide residues are still present in the colony at this time, the monitoring should be extended in the new season. A residue analysis of beekeeping matrices would enable one to know when the exposure of the colonies to the AI has occurred over the winter. It should be noted that these are test conditions. In reality, colonies might be exposed to larger amounts of AI over longer periods or to a mixture of AI.