Material and method of a semi-field brood test

1) Similar as for standard studies based on the EPPO 170 guideline; small healthy honey bee colonies are initially placed in tunnel tents (herein after named tunnels) shortly before full flowering of the crop, a few days before application of the test chemical.

2) Following exposure of the bees in the tunnel for the period of flowering of the crop (e.g. at least 7 days after application of the product), the hives are placed outside the tunnels for the remaining time of the study and are free to forage in the field.

3) It is important to check that the neighbouring environment within a radius of 3 km is free from bee attractive main crops (e.g. sunflower, maize, oil seed rape, fruit orchards) as well as the test substance or other compounds.

4) Mortality of honey bees, flight activity (Human et al., 2013), and condition of the colonies and development of the bee brood (Delaplane et al., 2013b) are evaluated several times over a period of at least 4 weeks after the initial brood assessment.

5) Results are evaluated by comparing the treated colonies with the water-treated colonies and with the reference chemical-treated colonies. Assessments The total observation period of the colonies is at least 28days.