Design of the test

1) A test includes at least 3 treatments:

- Test chemical

- Reference chemical or positive reference: An IGR known to produce adverse effects on honey bee brood (e.g. Fenoxycarb (CAS. 121-75-5), rate: at least 150 g/ha)

- Control: The plants are treated with tap water (water volume: 200-400 L/ha in case of Phacelia as test plant)

2) All spray applications should be done with the same water volume. It is suggested to run the test with at least three replicates for better statistical analysis. Thus, in total at least nine tunnels are established for one test. However, it is also possible to increase the number of replicates to four per treatment group in order to increase the stability of the test.